Figure 1
From: CASPER: context-aware scheme for paired-end reads from high-throughput amplicon sequencing
Overlap between paired-end reads. Next-generation sequencing techniques including the Illumina platform tend to result in rapid degradation of the sequencing quality as the end of a read is approached [12]. Consequently, the overlapping region (formed by the ends of forward and reverse reads) in a paired-end read frequently contains errors originating from sequencing and/or base-calling.