Figure 4From: Alternative splicing detection workflow needs a careful combination of sample prep and bioinformatics analysisTranscript coverage and FPKM in LSPs with 100-1000 ng input of total RNA. A, B, C) Coverage for transcripts detected only by tss, ss, ts1000 LSPs is much lower than the coverage of transcripts in common with ts100. Transcripts detected in common with respect to ts100 show nearly identical coverage. FPKM and coverage were calculated at transcript level using Cufflink (2.2.0)Back to article page