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Figure 6 | BMC Bioinformatics

Figure 6

From: Alternative splicing detection workflow needs a careful combination of sample prep and bioinformatics analysis

Figure 6

Benchmark dataset. A) Three datasets (20TS, 40TS, 80TS), based on spike-in of TruSeq (input: 100 ng total RNA) reads extracted respectively from 20, 40 and 80 million reads were generated using a common background made by 5 different TruSeq library preps having as input 100 ng total RNA (T) and 5 different TruSeq library preps having as input 1000 ng total RNA (C). B) Three datasets (20NU, 40NU, 80NU), based on spike-in of NuGEN (input: 2 ng total RNA) reads extracted respectively from 20, 40 and 80 million reads were generated using the common background described above. Synthetic spikes-in are present both in A and B.

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