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Table 1 Library preparation information

From: Alternative splicing detection workflow needs a careful combination of sample prep and bioinformatics analysis

 

RNA selection

RNA concentration (ng)

 

Poly A +

rRNA depletion

0.5

2

100

1000

1500

NuGEN v2

  

nu05

nu2

nu100

  

ScriptSeq v1

✓

     

ss

TruSeq unstranded

✓

   

ts100

ts1000

 

TruSeq stranded

✓

✓

  

tss (PolyA+) tss_total (rRNA depl)