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Figure 3 | BMC Bioinformatics

Figure 3

From: Simulation of DNA array hybridization experiments and evaluation of critical parameters during subsequent image and data analysis

Figure 3

Spot shifting. Every spot was shifted randomly relative to the ideal grid position by a Gaussian distributed distance with a given standard deviation σ. (A) simulated image, σ = 0.1824 mm, (B) Pearson correlation of simulated and calculated intensities dependent on the standard deviation of the spot centers from their ideal grid nodes, (C) mean distance between the calculated and the simulated spot centers dependent on the standard deviation of the spot centers from their ideal grid nodes. The vertical lines in (B) and (C) correspond to the image in (A). In (B) and (C) each point in the plot is determined by a single analysis of a simulated image, respectively.

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