Figure 3

Comparison of specific oligos suggested by ARB and PROBE for Thermotoga maritima, in comparison to the whole SSU database. A) Oligo suggested by ARB, but found to have lower than 70% melting probability in two other species. This was therefore rejected by PROBE because of insufficient specificity. B) Oligo suggested by ARB, but found to have lower than 70% melting probability when outlooping is considered. This was therefore also rejected by PROBE because of insufficient specificity. C) Oligo suggested by both programs, whereby the best outgroup matches have a higher than 70% melting probability.