Detection of nuclei in 4D Nomarski DIC microscope images of early Caenorhabditis elegans embryos using local image entropy and object tracking

Table 2 Specificity of nuclear detection by low-entropy regions selected by advanced forward and backward trackings

	T_f	T_t
		1 pixel^†		4%		8%		12%		16%		20%
Forward tracking	1 pixel^‡	0.48	(0.06)	0.54	(0.11)	0.56	(0.11)	0.56	(0.10)	0.58	(0.10)	0.59	(0.10)
	30%	0.57	(0.08)	0.69	(0.11)	0.74	(0.11)	0.78	(0.10)	0.84	(0.09)	0.87	(0.07)
	50%	0.66	(0.11)	0.79	(0.11)	0.84	(0.08)	0.87	(0.07)	0.90	(0.06)	0.94	(0.03)
	70%	0.74	(0.12)	0.86	(0.08)	0.90	(0.07)	0.93	(0.05)	0.94	(0.05)	0.97	(0.03)
	90%	0.83	(0.10)	0.93	(0.04)	0.95	(0.03)	0.98*	(0.01*)	0.99*	(0.01*)	1.00*	(0.00*)
Forward and backward trackings	1 pixel^‡	0.56	(0.08)	0.62	(0.13)	0.64	(0.13)	0.65	(0.12)	0.67	(0.13)	0.69	(0.13)
	30%	0.70	(0.10)	0.83	(0.12)	0.87	(0.09)	0.90	(0.07)	0.93	(0.05)	0.95	(0.03)
	50%	0.82	(0.12)	0.91	(0.07)	0.93	(0.05)	0.95	(0.05)	0.97	(0.04)	0.98	(0.02)
	70%	0.87	(0.11)	0.96	(0.05)	0.97	(0.04)	0.99	(0.02)	0.99	(0.01)	1.00	(0.00)
	90%	0.93	(0.08)	0.99	(0.01)	1.00	(0.00)	1.00*	(0.00*)	1.00*	(0.00*)	1.00*	(0.00*)

Data are mean and (SD) for five sets of 4D DIC microscope images of a C. elegans embryo from the one- to 24-cell stages.
*Data are for four sets of DIC microscope images, because no low-entropy regions were selected in one image set.
† Low-entropy regions were selected when they shared at least one pixel with regions already selected in the same focal plane at an adjacent time point, i.e., the same condition as used for the original forward and backward trackings.
‡ Low-entropy regions were selected when they shared at least one pixel with regions already selected in an adjacent focal plane at the same time point, i.e., the same condition as used for the original forward and backward trackings.

ISSN: 1471-2105