Quantitative analysis of EGR proteins binding to DNA: assessing additivity in both the binding site and the protein

Table 4 Absolute K_a(10⁶M^-1) for six DNA binding sites and six variants of zif268, derived from the combination of Table 2 and Table 3.

DNA\Prot	RE	QE	DE	RN	QN	DN
CG	406	3.0	2.5	81	7.4	1.2
CA	109	4.5	2.8	30	3.5	1.4
CC	33	6.4	4.7	33	3.9	3.1
AG	63	3.9	3.6	105	33	17
AA	26	4.0	3.7	56	18	4.8
AC	16	5.7	5.5	77	21	14

ISSN: 1471-2105

DNA\Prot	RE	QE	DE	RN	QN	DN
CG	406	3.0	2.5	81	7.4	1.2
CA	109	4.5	2.8	30	3.5	1.4
CC	33	6.4	4.7	33	3.9	3.1
AG	63	3.9	3.6	105	33	17
AA	26	4.0	3.7	56	18	4.8
AC	16	5.7	5.5	77	21	14

DNA\Prot	RE	QE	DE	RN	QN	DN
CG	406	3.0	2.5	81	7.4	1.2
CA	109	4.5	2.8	30	3.5	1.4
CC	33	6.4	4.7	33	3.9	3.1
AG	63	3.9	3.6	105	33	17
AA	26	4.0	3.7	56	18	4.8
AC	16	5.7	5.5	77	21	14

DNA\Prot	RE	QE	DE	RN	QN	DN
CG	406	3.0	2.5	81	7.4	1.2
CA	109	4.5	2.8	30	3.5	1.4
CC	33	6.4	4.7	33	3.9	3.1
AG	63	3.9	3.6	105	33	17
AA	26	4.0	3.7	56	18	4.8
AC	16	5.7	5.5	77	21	14