Table 1 The different sub-classes of bad spots.
From: MASQOT: a method for cDNA microarray spot quality control
Class | Description |
|---|---|
not bad | No issue. Contains all spots with no apparent problems according to the classification by the three experienced users. |
HIFI | High-Intensity Foreground Issue. Typically intensity distribution issues, such a dye debris in the foreground region or donut-shaped spots, with very distinct characteristics. |
LIFI | Low-Intensity Foreground Issue. Weak intensity distribution issues in the foreground region or morphological issues. |
HIBI | High-Intensity Background Issue. Typically intensity distribution issues, such a dye debris in the background region, with very distinct characteristics. |
LIBI | Low-Intensity Background Issue. Weak intensity distribution issues or faint increases in noise level in the background region. |
HIFI/HIBI | A combination of HIFI and HIBI. |
HIFI/LIBI | A combination of HIFI and LIBI. |
LIFI/HIBI | A combination of LIFI and HIBI. |
LIFI/LIBI | A combination of LIFI and LIBI. |
HIFI/LIFI | A combination of HIFI and LIFI. |
HIFI/LIFI/HIBI | A combination of HIFI and LIFI and HIBI. |