Utilization of two sample t-test statistics from redundant probe sets to evaluate different probe set algorithms in GeneChip studies

Table 3 Genes and primer sequences for qRT-PCR.

Gene	GenBank	Primer sequences	Fold change (diabetic vs. normal)
GSH-ST	J03914	Forward: GATGTCCTTGATCAACACCG Reverse: GGCCGCTCTTCATGTAGTCAG	5.47
MHC	x04267	Forward: GTCAAGTCCTACAAGAGGCAGG Reverse: GATTCTGCAATATCCGCACG	4.78
Metallothionein 1 and 2	M11794	Forward: CTGCGGCTGCAAGAACTG Reverse: CTTGTCCGAGGCACCTTTG	7.21
HMGCoAS	M33648	Forward: CTGCCCAAACGTCTAGACTCC Reverse: GAAGAGGTTGCTTGTGTCACC	6.2
ALS	03190	Forward: GCTTATGAGGCAGATGCTAATGG Reverse: ATGCCTGGTCATCAACTCATC	-2.96
PHAS-1	U05014	Forward: ACTAGCCCTACCAGCGATGAG Reverse: TGTCCATCTCAAACTGTGACTC	2.64
DiEcoAR	D00569	Forward: TGCAGTGATTATGCCTCTTGG Reverse: CTTCGATTACATCCCACTCCTC	2.58
CPT-1	L07736	Forward: TTGTCTACGAGCCAGACTCCTC Reverse: AGGAGACACCATAGCCGTCATC	2.42
RAD	U12187	Forward: CATCCTAGTGGGCAACAAGAG Reverse: AGGTCTCGATGAACTTGCAGTC	1.53
CK	X59736	Forward: CTGGTCGCTACTACAAGCTGTC Reverse: CCCAGCGCATGTTAGTAAAGG	-3.93
β-2-microglobulin	Y00441	Forward: CCACCGAGACCGATGTATATG Reverse: CGGATCTGGAGTTAAACTGGTC

In the order the genes are glutathione S-transferase, embryonic skeletal muscle myosin heavy chain, metallothionein-1 and 2, mitochondrial 3-hydroxy-3methylglutaryl-CoA synthase, 5-aminolevulinate synthase, PHAS-I protein, 2,4-dienoyl-CoA reductase, carnitine palmitoyltransferase I, Ras-related protein, and sarcomeric mitochondrial creatine kinase.

ISSN: 1471-2105