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Table 5 Correlation between microarray and RT-PCR data. Pearson correlation of expression values between six microarray algorithms and three Real Time RT-PCR algorithms on a subset of 18 genes. The negative sign from the numbers in the second and last column are removed, because a lower ΔCt value means more gene product present, consequently leading to a negative correlation with microarray data. All correlations were significant. ns = not significant (p < 0.01). * = p < 0.05, ** = p < 0.01

From: How to decide? Different methods of calculating gene expression from short oligonucleotide array data will give different results

 

ΔCt

Assumption Free

ΔCt + Assumption Free

MAS 5.0

0.515**

0.311*

0.406**

dChip PMMM

0.468**

0.298*

0.382**

dChip PM

0.460**

0.300*

0.397**

RMA

0.554**

0.378**

0.459**

GC-RMA

0.495**

0.297*

0.407**

PDNN

0.462**

0.252ns

0.386**