Table 6 RT-PCR data of RMA specific genes. Ethylene/air expression signals from 10 genes calculated with RMA and compared to the Real Time RT-PCR data as calculated with ΔCt. All RMA signals from ethylene treated plants are significantly (0.05 > p > 0.01) different from the air controls (average p = 0.037). The Real Time RT-PCR data shown, are averages, standard deviations and p values after comparison with the control. The average control signal is set to 1. The numbers in bold indicate genes which show a change in expression similar for both methods. For RMA, n = 3 and for Real Time RT-PCR, n = 3–5.

average

average

stdev

p

0.65

0.83

0.075

0.456

0.72

0.76

0.135

0.181

0.73

0.82

0.089

0.155

0.78

0.54

0.109

0.021

1.29

0.99

0.134

0.958

1.31

0.72

0.104

0.065

1.35

1.22

0.523

0.700

1.44

0.84

0.066

0.270

1.51

0.72

0.227

0.356

1.99

0.96

0.132

0.804