Table 3 Sequences of real-time quantitative RT-PCR primers and probes of high-, medium- and low-abundance gene transcripts in rats
From: Biologically relevant effects of mRNA amplification on gene expression profiles
Gene | Primer | Sequence (5'→3') | Position |
|---|---|---|---|
Cyclophilin A (M19533) (high) | F | GGGAGAAAGGATTTGGCTATAAGG | 167–190 |
|  | Probe | TGAAGTCACCACCCTGGCACATGAAT | 219–244 |
|  | R | GCCACCAGTGCCATTATGG | 249–267 |
Ribosomal Protein S9 (NM_031108) (medium) | F | CTCGACCAGGAGCTAAAGTTGATT | 99–122 |
|  | Probe | ACACCTCACGTTTGTTCCGGAGTCCATACT | 127–156 |
|  | R | TCTTCGCCAGGGTAAATTTGA | 163–183 |
Collagen VI alpha 3 (XM_346073) (low) | F | CAGGAGGACCGAGAGCTCAT | 7630–7649 |
|  | Probe | ATGCCTTGCAGATCAATAACACAGCAGTAGG | 7652–7682 |
|  | R | CCCTCCTTGCAGGCAGAAC | 7693–7711 |