Automated in-silico detection of cell populations in flow cytometry readouts and its application to leukemia disease monitoring

Table 1 Sample characteristics. Sample No.: Sample Number, Patient No.: Patient Number (S: custom-built mix samples), Day: Day of treatment (d0: before initial treatment, d8: after first week of treatment, d15: after second week of treatment), Source: Source of sample (BM: bone marrow, PB: peripheral blood, Co: control), Man.% Leukemic: Percentage of events deemed leukemic by manual gating. SVM % Leukemic: Percentage of events deemed leukemic by SVM prediction.

Sample No.	Patient No.	Day	Source	Man. % Leukemic	SVM % Leukemic
1	I	d8	PB	5.88	5.88
2	II	d8	PB	0.04	0.03
3	III	d0	BM	66.33	64.28
4	III	d0	PB	68.09	67.92
5	III	d8	PB	0.26	0.32
6	IV	d0	BM	82.28	82.05
7	IV	d0	PB	6.23	6.12
8	V	d0	BM	31.83	30.82
9	V	d0	PB	13.76	13.17
10	III	d15	BM	0.00	0.03
11	IV	d8	PB	0.68	0.66
12	V	d8	PB	0.08	0.08
13	VI	d0	BM	86.32	88.13
14	VI	d0	PB	56.59	56.97
15	VII	d0	BM	82.41	82.63
16	VII	d0	PB	44.67	44.64
17	VIII	d0	BM	82.87	91.31
18	VIII	d0	PB	59.34	62.34
19	IX	d0	BM	78.59	78.44
20	IX	d0	PB	41.81	41.76
21	X	d0	BM	38.18	37.63
22	X	d0	PB	8.93	9.15
23	IV	d15	BM	0.22	0.50
24	V	d15	BM	0.22	0.27
25	VIII	d8	PB	0.33	0.47
26	VII	d8	PB	0.96	0.97
27	VI	d8	PB	0.46	0.53
28	IX	d8	PB	0.93	1.02
29	XI	d0	BM	29.35	30.05
30	XI	d0	PB	11.24	11.13
31	X	d8	PB	0.81	0.82
32	VIII	d15	BM	0.48	0.40
33	VII	d15	BM	0.55	0.52
34	VI	d15	BM	0.19	0.21
35	IX	d15	BM	1.01	1.02
36	X	d15	BM	0.41	0.49
37	XI	d15	BM	0.02	0.02
38	s	Co		0.00	0.01
39	s	Co		100.00	97.12
40	s	0.01		0.01	0.01
41	s	0.1		0.10	0.08
42	s	1.0		1.00	0.82
43	s	10		10.00	9.41

ISSN: 1471-2105