Figure 5From: Improvement in the Reproducibility and Accuracy of DNA Microarray Quantification by Optimizing Hybridization ConditionsDistribution of log2 expression ratios. Labeled mouse liver RNA (Cy5) and Universal Mouse Reference RNA samples (Cy3) were hybridized with MWG mouse 20 K using GlassHyb™ buffer or 5× SSC, 0.1% SDS buffer with different concentrations of formamide: A. GlassHyb™ buffer. B. 27% formamide. C. 35% formamide. D. 40% formamide. The log2 expression ratio (liver/reference) is plotted on the x-axis and the probability is on the y-axis.Back to article page