Figure 7

"In silico PCR" and "Probe alignment" tools infast DB. (A) The "in silico PCR" option in fast DB. (1) Multi-alignment of transcript sequences of the human RANPB9 gene, exon by exon. (2) Explicit skipping of exon 5 by the [GenBank:BC063849] transcript shown on multi-alignment. (3) Direct selection of primers on multi-alignment. (4) Form for inputting primer sequences. (5) General information regarding input primers: length, GC percentage, TM, location and sequence. (6) Link to test the user's primers with ASePCR. (7) Expected PCR product for each transcript with its length and the link to its sequence. (8) Sequence of one expected PCR product, clickable in order to display the restriction enzyme cut sites for this sequence. (B) The "probe alignment" option in fast DB. (1) Graphical representation of probe sequences aligned with the genomic exon/intron structure. (2) Table with alignment information for all aligned probe sequences; data regarding the selected probe are highlighted in red. (3) Alignment of the selected probe sequence with the genomic sequence.