Figure 3

Expansion of the (a) osmolyte and (b) biogenic amine portions of the phylogenetic tree. Seven of the 46 osmolyte sequences remain experimentally uncharacterized (indicated by arrows). Annotations of the remaining sequences are indicated. Using a phylogenomic approach, we assign functions to five of the seven. The remaining pair (indicated by question marks) is still without functional annotation. Similarly, there are five uncharacterized sequences within the biogenic amine subfamily; we have assigned function to all five. Branch points 1 and 2 designate the differentiation of the two GABA symporter out-groups. Color differences within each out-group correspond to functional distinctions discussed within the text.