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Table 5 Reactions in the anneal step incorporated in the basic model

From: Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process

 

[A] + [Q] ↔ [AQ]

 

[AQ] + [P] ↔ [AQP]

 

[A'] + [P'] ↔ [A'P']

 

[A'] + [A] → [A'A]