Ref (v1, v6, v7, v19, v20, v22) | G (no v22) | F (no v20) | E (no v19) | B (no v6) | A (no v1) | C (no v7) | I (no v20v22) | H (no v19v22) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Reactions d | MIa [b] | MI [a] | [%c] | MI [b] | [%] | MI [b] | [%] | MI [b] | [%] | MI [b] | [%] | MI [b] | [%] | MI [b] | [%] | MI [b] | [%] |
1: G→G6P | 0.267e | 0.267 | - | 0.267 | - | 0.267 | - | 0.267 | - | x | - | 0.267 | - | 0.267 | - | 0.267 | - |
2: G6P→G3P+DAP | 0.367 | 0.387 | 5% | 0.628 | 71% | 0.541 | 47% | 0.398 | 8% | x | - | 0.627 | 71% | 0.628 | 71% | 0.572 | 56% |
3: G6P→R5P+CO2 | 0.131 | 0.199 | 53% | 0.526 | 303% | 0.340 | 160% | 0.131 | 0% | 0.131 | 0% | 0.401 | 207% | 0.526 | 303% | 0.383 | 193% |
4: DAP→G3P | 0.367 | 0.387 | 5% | 0.628 | 71% | 0.541 | 47% | 0.398 | 8% | x | - | 0.627 | 71% | 0.628 | 71% | 0.572 | 56% |
5: G3P→Pyr | 0.735 | 0.774 | 5% | 1.256 | 71% | 1.082 | 47% | 0.795 | 8% | x | - | 1.253 | 71% | 1.256 | 71% | 1.144 | 56% |
6: Pyr→L | 0.475 | 0.475 | - | 0.475 | - | 0.475 | - | x | - | 0.475 | - | 0.475 | - | 0.475 | - | 0.475 | - |
7: Pyr+Glu→A+aKG | 0.100 | 0.100 | - | 0.100 | - | 0.100 | - | 0.100 | - | 0.100 | - | 1.488 | inf | 0.100 | - | 0.100 | - |
8: Pyr→ACA+CO2 | 1.031 | 1.031 | 0% | 1.562 | 51% | 1.901 | 84% | x | - | x | - | 0.957 | -7% | 1.562 | 51% | 1.906 | 85% |
9: Oxa+ACA→Cit | 1.031 | 1.031 | 0% | 1.562 | 51% | 1.901 | 84% | x | - | x | - | 0.957 | -7% | 1.562 | 51% | 1.906 | 85% |
10: Cit→aKG+CO2 | 1.031 | 1.031 | 0% | 1.562 | 51% | 1.901 | 84% | x | - | x | - | 0.957 | -7% | 1.562 | 51% | 1.906 | 85% |
11: aKG→Mal+CO2 | 1.156 | 1.156 | 0% | 1.604 | 39% | 2.532 | 119% | x | - | x | - | 1.443 | 25% | 1.604 | 39% | 2.530 | 119% |
12: Mal→Oxa | 0.994 | 0.994 | 0% | 1.398 | 41% | 1.769 | 78% | x | - | x | - | 1.093 | 10% | 1.398 | 41% | 1.769 | 78% |
13: Mal→Pyr+CO2 | 0.209 | 0.240 | 15% | 0.352 | 68% | 0.920 | 341% | 0.209 | 0% | 0.209 | 0% | 0.903 | 332% | 0.352 | 68% | 0.918 | 340% |
14: Oxa+Glu→Asp+aKG | 0.131 | 0.199 | 53% | 0.526 | 303% | 0.340 | 160% | 0.131 | 0% | 0.131 | 0% | 0.401 | 207% | 0.526 | 303% | 0.383 | 193% |
15: Glu→aKG+NH4 | 0.150 | 0.182 | 21% | 0.298 | 98% | 0.870 | 479% | 0.150 | 0% | 0.150 | 0% | 0.586 | 289% | 0.298 | 98% | 0.870 | 479% |
16: Q→Glu+NH4 | 0.117 | 0.145 | 23% | 0.325 | 177% | 0.553 | 372% | 0.117 | 0% | 0.117 | 0% | 0.569 | 386% | 0.325 | 177% | 0.548 | 367% |
17: R5P+Asp+Q→Pu | 0.104 | 0.277 | 165% | 0.293 | 181% | 0.200 | 91% | 0.104 | 0% | 0.104 | 0% | 0.225 | 116% | 0.526 | 404% | 0.383 | 267% |
18: R5P+Asp+2Q→Py | 0.078 | 0.132 | 69% | 0.283 | 262% | 0.177 | 126% | 0.078 | 0% | 0.078 | 0% | 0.209 | 168% | 0.263 | 237% | 0.163 | 108% |
19:→NH4 | 0.141 | 0.141 | - | 0.141 | - | 1.419 | 904% | 0.141 | - | 0.141 | - | 0.141 | - | 0.141 | - | 1.412 | 899% |
20:→Q | 0.132 | 0.132 | - | 1.127 | 752% | 0.132 | - | 0.132 | - | 0.132 | - | 0.132 | - | 1.107 | 737% | 0.132 | - |
21:→CO2 | 3.338 | 3.338 | 0% | 4.770 | 43% | 6.966 | 109% | x | - | x | - | 3.843 | 15% | 4.770 | 43% | 6.966 | 109% |
22: Pu-Py (constraint) | 0.100 | 0.354 | 254% | 0.100 | - | 0.100 | - | 0.100 | - | 0.100 | - | 0.100 | - | 0.526 | 426% | 0.383 | 283% |
Mean | 0.554 | 0.587 | 39% | 0.899 | 155% | 1.138 | 196% | 0.217 | 2% | 0.156 | 0% | 0.802 | 122% | 0.927 | 180% | 1.168 | 214% |
Measured fluxes [number] | 6 | 5 | 5 | 5 | 5 | 5 | 5 | 4 | 4 | ||||||||
Estimated fluxes [number] | 16/16 | 17/17 | 17/17 | 17/17 | 7/17 | 10/17 | 17/17 | 18/18 | 18/18 | ||||||||
<25% (·Ref) | 12 | 0 | 0 | 10 | 7 | 5 | 0 | 0 | |||||||||
25–100% (<2·Ref) | 3 | 11 | 8 | 0 | 0 | 4 | 11 | 7 | |||||||||
100–300% (2–4·Ref) | 2 | 3 | 5 | 0 | 0 | 5 | 2 | 7 | |||||||||
>300% (>4·Ref) | 0 | 3 | 4 | 0 | 0 | 3 | 5 | 4 |