Figure 1

Representative real-time PCR results for transgene copy number quantification. A, The amplification plot for external standard curve method, where both serial diluted external control samples and transgene sample amplification curves are shown. B. The amplification plot for internal reference gene method, where transgene copy number equals to that of reference gene as shown by the overlapped amplification curves. C, The amplification plot for internal reference gene method, where transgene copy number does not equal to that of reference gene as shown by the separated amplification curves. D. The amplification plot for internal reference gene method, where non-transformants were tested as shown by the extremely large Ct numbers for transgene.