Figure 5

Pairwise comparison of stable individuals. Pairwise comparison of all sequences with highest stability (σ = 1) and wild-type VH (individual 6) with respect to structure (left) and sequence (right). The left panel shows the pairwise RMSD with respect to C _α -positions after optimal superposition; color codes RMSD in units of 1 Å (= 0.1 nm). The right panel gives the percentage sequence identity between the same peptides as on the left (using same numbering of individuals as there). The figure shows that the individuals fall into two clusters or sub-folds, a cluster with modified fold and sequences (individuals 1–5) and a cluster similar to the wild-type (individuals 6–10).