Bioinformatic analyses of mammalian 5'-UTR sequence properties of mRNAs predicts alternative translation initiation sites

Table 4 Significant Parameters Utilized by C4.5 Classification Tree as Properties to Determine aTIS

Critical parameters in the classification tree are illustrated with respect to their average values for alternative start sites and non-alternative start sites. Differences among these values facilitated the effective classification of mRNAs with aTIS. The number of AUGs is a count of the number of AUGs in the 5'-UTR. Consensus sequences matches against a C in the -7 position from the start site and a G or C in the -6 position. The sequences are also scanned for the IRES secondary structure and the ratio of Guanine and Cytosine in the 5' UTR is also measured. Although the G/C ratio was the least important variable, it resulted in a split in the classification tree and is, therefore, listed as an parameter required for the final classification results.

ISSN: 1471-2105