Figure 6
Real-time PCR amplification plots obtained from the same starting DNA in the presence of IgG acting as reaction inhibitor. This inhibition system produces curves which are progressively less steep than non-inhibited reactions with increasing IgG concentrations (A). When analysed by the Ct, Cp and Cy 0 methods these curves showed a RE% of -25.37%, -9.02% and 4.98% and a CV% of 25.62%, 10.66% and 4.33%%, respectively (B).