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Figure 1 | BMC Bioinformatics

Figure 1

From: AccPbFRET: An ImageJ plugin for semi-automatic, fully corrected analysis of acceptor photobleaching FRET images

Figure 1

Screenshot of an example analysis with the AccPbFRET plugin. The ImageJ (left top) and AccPbFRET (right) dialog windows are displayed, together with donor channel source images taken by a confocal microscope (labeled appropriately as 'Donor before bleaching', 'Donor after bleaching'), as well as the calculated, corrected FRET image ('Transfer image'), on which ROIs can be selected and statistics calculated. These statistics can be seen in a separate 'Results' window, and a histogram of the FRET distribution is also presented.

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BMC Bioinformatics

ISSN: 1471-2105

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