Figure 2From: AccPbFRET: An ImageJ plugin for semi-automatic, fully corrected analysis of acceptor photobleaching FRET imagesThe analysis process. In this figure, the changes of donor and acceptor images during the steps of the analysis process are shown. DB, DA: donor images before and after bleaching the acceptor; AB AA: acceptor images before and after bleaching (same images as in Figure 1, cropped to fit the page). STEP1: original images; STEP2: images after registration (note the disappearance of the top lines from DA and AA); STEP3: after background subtraction; STEP4: after Gaussian filtering; STEP 5: thresholded images. The corrected FRET/transfer image and histogram derived from it are also displayed.Back to article page