Figure 7
From: Position dependent mismatch discrimination on DNA microarrays – experiments and model
Influence of the synthesis error rate on the shape of the single base mismatch defect profile. The defect profiles (which correspond to the experimental data in Fig. 3 (A) and (B)) were calculated for error rates between 0 and 20 percent (per nucleotide coupling step). In (A) a positional influence is rather independent of the error rate – the duplex free energy range covered by the defect profile is within the approx. linear transition region. Whereas in (B) at an error rate of 0 percent, the free energy range of the defect profile doesn't match the transition region – the positional influence is hardly visible. At larger error rates the positional influence becomes dominating over the defect-type related influence.