Fig. 2

Comparative analysis of SPECtre against previously published translational classification algorithms. a Performance of SPECtre, RiboTaper, FLOSS and ORFscore classification of ORF translation at various RPF abundance cutoffs as measured by area under the curve (AUC) in ribosome profiling of HEK293 cells [4]. b Receiver operating characteristic (ROC) curves of SPECtre, RiboTaper, FLOSS, and ORFscore at a cutoff of 1.0 FPKM. c Performance of SPECtre, FLOSS, and ORFscore classification of ORF translation in ribosome profiling of mESC [7] at various RPF abundance cutoffs as measured by AUC. d Performance of SPECtre, FLOSS, and ORFscore classification of ORF translation in a meta-analysis of ribosome profiling in zebrafish [2] over various RPF abundance cutoffs as measured by AUC. All SPECtre analyses were based on 30 nt sliding windows, using a step size of three between each window