Fig. 1

Schematic representation of RiboTagger detection scheme. Starting with shotgun sequencing reads from (either gDNA or cDNA) RiboTagger procedes as follows; (1) all reads are screened using PSSMs for the presence of a conserved recognition sequence (blue arrowed rectangles) adjacent to V-regions using a cohort of pre-defined recognition profiles; (2) for reads that are positive for recognition sequences, the adjacent V-region tag sequence is extracted, assuming if sufficient length is available (dashed blue rectangles denote in sufficient length in the tag sequence); and (3) for related tag sequences, equivalent coordinates are defined, prior to counting and annotation