Fig. 1

a The “3D” analysis pipeline of next-generation sequencing based TCR repertoire data. It consists of assessing the Diversity of the T-cell repertoire, evaluating the Dynamics of T-cell clonotypes across the time course or among different biological compartments, performing Differential testing to investigate differences in the abundance of each clonotype between pre- and post-treatment. b The count distribution of unique TCR clonotypes of a healthy subject (NeoACT study). Using one of the healthy subjects for illustration, the x-axis represents each unique clonotype in descending order of the count, and the y-axis is log₁₀(count) of each clonotype from PBMC at week 0 (black), week 2 (red) and week4 (purple). c The count distribution of unique TCR clonotypes of a treated prostate cancer subject (NeoACT study)