Fig. 5From: Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experimentsScore Comparisons. Each screenshots depict side-by-side plots containing all guides targeting the MCDS of MUC4. a–b Plotting Doench 2016 Full and Positionless against percent peptide reveals a scoring bias against guides targeting the 3′ end of the MCDS in Doench 2016 Full. c Guide Picker can also be used to illustrate score distribution patterns for different scores. In these plots, Doench 2016 Positionless guide scores cluster toward the <50 end of the plot while Full guide scores tend to cluster around 50. d This is demonstrated even more clearly using Force Layout modeBack to article page