Fig. 2

Workflow of the nuclear spatial pattern analysis pipeline. 1 The pipeline takes a multi-tiff time series stack as input. 2 The muscle cell boundary is extracted using FMAj tool. 3 The colour channel which contains nuclei is segmented to obtain the nuclear regions. The muscle boundary is used to remove nuclei which lie outside the muscle cell. The external nuclei (present inside the fragments of dead muscle) are identified and removed from the segmentation results to avoid incorrect feature calculations. 4 & 5 Nuclear spatial pattern features are calculated using the extracted nuclear regions and these features are used for time series statistical analysis of the myonuclear distributions and localization during metamorphosis