Fig. 2

Multitrack wave files representing a portion of an auditory display. These tracks play in unison to generate the auditory display and each represent approximately 80 nucleotides beginning at nucleotide position 65. This sequence is located in the 5′ untranslated region and includes a TRS region and a uORF. Each audio stream was generated from a different algorithm, only nucleotides that gave rise to audio are shown (the entire nucleotide sequence is shown in track 2). In track 1, each nucleotide generates a note for every beat unless it is a repeat of the previous in which case the length of the note is extended. In track 2, each di-nucleotide generates a note every second beat. In tracks 3 and 4, audio from the GC track is only triggered when the GC ratio changes by an increment of 0.1. Each change in the GC ratio is indicated by a plus (+) or minus (−) symbol on the wave files. In track 5, only codon sequences beginning with a start codon (AUG) are shown through to the next stop codon (e.g. UAA). Isolated stop codons also give rise to a note. This track is a compilation of audio form three sub-tracks each representing a different reading frame and notes in this track are panned left, centre or right, respectively. Track 6 represents the audio generated from metadata that indicates the location of a TRS region. Additionally, the consensus sequence within this region is coloured purple in the visual display. Track 7 represents audio generated by the occurrence of three nucleotides of the same type. Other data tracks are not represented since no audio was generated in these during processing of this sequence of the genome. Additionally, the amino acid sequence of the ORF is shown in the codon track 5