Fig. 1

Robustness of AStruct in predicting allele-specific structure events. a Flowchart of AStruct pipeline. b ROC curves of simulated datasets under different sequencing depths. c The AStruct score distribution of all heterozygous SNPs in six cell lines (H9, HEK293, HEK293T, HeLa, HepG2, and K562). d The allele level reactivity score derived from the icSHAPE data of K562 cell line for three SNPs representing the three AStruct group (Low: rs1292926445; Medium: rs12133138; High: rs17424311). The P values indicating the difference in reactivity score between two alleles for each base were shown in the top. e The correlation of ASRS SNPs between different cell lines. The value in top-right triangle presents the number of common ASRS SNPs; the value in bottom-left triangle presents the Pearson correlation coefficient of the AStruct scores. Background color presents the P Value, where gray indicates P > 0.05. f Distribution of ASRS SNPs in different genomic regions (3′UTR, CDS, 5′UTR, intron, and ncRNAs). Left: Stacked bar plot for three AStruct groups. Right: Comparison the sites number of different regions between different groups (Fisher’s exact test). The number represents the Odds Ratio. Background color represents the significant P Value