Fast-Find: A novel computational approach to analyzing combinatorial motifs
© Hamady et al; licensee BioMed Central Ltd. 2006
Received: 28 July 2005
Accepted: 4 January 2006
Published: 4 January 2006
Many vital biological processes, including transcription and splicing, require a combination of short, degenerate sequence patterns, or motifs, adjacent to defined sequence features. Although these motifs occur frequently by chance, they only have biological meaning within a specific context. Identifying transcripts that contain meaningful combinations of patterns is thus an important problem, which existing tools address poorly.
Here we present a new approach, Fast-FIND (Fast-F ully I ndexed N ucleotide D atabase), that uses a relational database to support rapid indexed searches for arbitrary combinations of patterns defined either by sequence or composition. Fast-FIND is easy to implement, takes less than a second to search the entire Drosophila genome sequence for arbitrary patterns adjacent to sites of alternative polyadenylation, and is sufficiently fast to allow sensitivity analysis on the patterns. We have applied this approach to identify transcripts that contain combinations of sequence motifs for RNA-binding proteins that may regulate alternative polyadenylation.
Fast-FIND provides an efficient way to identify transcripts that are potentially regulated via alternative polyadenylation. We have used it to generate hypotheses about interactions between specific polyadenylation factors, which we will test experimentally.
DNA- and RNA-binding proteins are essential for the regulation of gene expression at many levels. They control many biological processes in all organisms by altering gene expression at the levels of transcription, pre-mRNA splicing, mRNA export, stability, localization, and translation. Although some proteins bind specific sequences, others bind short or degenerate patterns, also called motifs, that occur frequently in the genome by chance. These patterns can even be defined by base composition rather than by an exact sequence.
Proteins that bind frequently-occurring sites cannot individually be highly specific, but such proteins can achieve specificity by cooperation in complexes clustered near regulatory sequences. This combinatorial control is the rule rather than the exception in higher eukaryotes for critical processes including transcription  and splicing , and has also been observed in bacterial transcription . Building up regulatory complexes in this way, rather than using individual gene- or transcript-specific factors, confers many advantages. These advantages include tissue-specific fine-tuning of biological responses through interactions with different combinations of proteins, increased evolutionary stability by mitigating deleterious effects of changes in an individual pattern, and repeated recognition of sequences by dynamic multi-protein complexes such as the spliceosome .
Unfortunately, this combinatorial flexibility substantially complicates computational searches for patterns involved in function, because most occurrences of most patterns are not biologically meaningful. Although there are many well-established approaches for defining and searching for patterns in strings, these techniques typically either require a linear scan of the sequence or cannot deal with compositionally-defined patterns. Examples of the former type include regular expressions (reviewed in ) and weight matrices (reviewed in ); examples of the latter include suffix trees and suffix arrays (reviewed in ). Because the patterns for nucleic acid binding proteins are often poorly defined, it is critical to avoid a linear-time search, and to be able to conveniently locate multiple patterns near regions of biological interest. In this paper, we present Fast-FIND (Fast-F ully I ndexed N ucleotide D atabase), a new algorithm that addresses this class of problem by indexing sequences in a relational database.
As an example of the application of Fast-FIND to the study of combinatorial regulation, we searched for patterns potentially involved in alternative polyadenylation. Polyadenylation, or addition of a poly(A) tail at the 3' end of a cleaved mRNA, is required for the synthesis of almost all mRNAs in higher eukaryotes [8, 9]. The polyadenylation machinery recognizes a combination of two patterns: a conserved AAUAAA consensus polyadenylation signal located between 10 and 30 nucleotides upstream of the cleavage site, and a relatively flexible GU-rich enhancer element located between 20 and 40 nucleotides downstream of the cleavage site [10, 11].
Alternative polyadenylation, in which the transcripts from a single gene have alternative 3' ends, is an important but poorly-studied process. Although both tissue-specific and disease-specific differences in alternative polyadenylation patterns have been reported [9, 10, 12–14], and many transcripts in different organisms have alternative 3' ends (50% in human, 31% in mouse, 28% in rat and 25% in Arabidopsis) , little is known about how this important process of gene regulation occurs. We chose to study alternative polyadenylation in Drosophila because this model organism can be conveniently manipulated by genetic techniques to confirm predictions from genome-wide sequence analysis.
Databases and programming
We downloaded sequences for Drosophila complementary DNAs (cDNAs)  and expressed sequence tags (ESTs)  in FASTA format. All source code for Fast-FIND, written in Python, is available from the authors upon request.
We implemented Fast-FIND in a relational database because relational database management systems (RDBMS) automatically provide solutions to many problems that would need to be considered if using custom data structures and code to support searches. These solutions include a standard, declarative language for performing queries (SQL), efficient indexing mechanisms and caching strategies, support for concurrent access by multiple users, and scalability to multiple processors and/or distributed systems .
Fast-FIND consists of two phases: a one-time, O(N) indexing phase, which generates and populates the database tables, and an O(log N) search phase, in which arbitrary queries can be evaluated. We now describe these phases in detail.
Fast-FIND indexing phase
During the Fast-FIND indexing phase, we analyze each sequence using overlapping 32-base sliding windows. We pack the bases in each window into a bitvector (an array of 1's and 0's, which allows us to perform efficient comparisons of strings by treating them as numbers) and store them in the database. We also use overlapping, variable-sized sliding windows to calculate the composition of each 3- to 20-base substring within the sequence. This indexing produces two ancillary tables. One table contains the bitvector and position of each 32 base window, while the other contains a window size, a pointer to the window composition, and the window position in the longer sequence. The bitvector table is used for exact matching, and the composition table is used for compositionally defined patterns.
For each unambiguous position N in the sequence we compute and store the base composition of all 3- to 20-base windows [N:N+y], where y is the length of the window (Figure 1b). We thus calculate and store the location and composition of all sliding windows from 3 to 20 bases in length within each sequence; all biologically relevant protein-binding sites in RNA that we are aware of fall within this range.
Fast-FIND search phase
Fast-FIND supports composition and exact searches by numerical range operations on the composition and sequence indexes respectively. It supports inexact searches (searches for a pattern defined using IUPAC degenerate symbols) using application logic rather than within the database.
To find a pattern of specified length and composition, we use range operations to look up all possible counts of each of the four bases that could match the pattern, and join these compositions to the table containing the locations of the windows that match them. This design allows us to rapidly locate arbitrarily degenerate patterns with a specified range of compositions.
Exact pattern searches
Degenerate pattern searches
We support searches using patterns containing any of the IUPAC degenerate bases using two methods, depending on the level of degeneracy. In the first method, we generate all possible sequences that match the pattern, and then combining the search results of exact matching from each sequence. Although this technique is exponential (O(4N)) in the number of unspecified positions, five degenerate bases (1024 combinations) are sufficient to identify essentially all well-characterized binding sites for RNA-binding proteins [20, 21] and imposes little computational burden in practice. In the second method, we use a different encoding scheme in which each position is represented by four bits, indicating presence/absence of each base in the degenerate symbol, and perform masked searches. This latter method requires a linear table scan, making it O(N) in the number of indexed windows, but is useful for highly degenerate searches and for searches against sequences containing ambiguous bases.
Applying Fast-FIND to alternative polyadenylation in Drosophila
Mapping cDNAs and ESTs onto the genome
We used BLAT  to map cDNAs and ESTs onto the genome, which also provided information about which ESTs matched which cDNAs (because both matched the same positions on the genome).
Identifying candidates for alternative polyadenylation
We defined cDNAs with alternative 3' ends as candidates for alternative polyadenylation. These candidates were identified by ensuring that at least two sets of ESTs annotated as "3prime" or "complete" matched somewhere on the same cDNA. The 'region of interest' for alternative polyadenylation is defined as the region within a candidate cDNA sequence that begins 100 nucleotides upstream of the end of the first EST set through the end of the cDNA (Figure 3). Sequences within this region were further indexed to support pattern searches as described above.
Properties of the alternative polyadenylation database
The relational database took ~2 hours to build on a 2.4 GHz Pentium 4 Dell Optiplex, a one-time investment because incremental updates can be used to add new data as necessary, and required ~2 GB (for both tables and indexes) of disk space. For comparison, the original EST library was 165 MB and the original cDNA library was 20 MB. From the matches between Drosophila cDNAs and ESTs, we determined the subset of cDNAs that showed evidence of potential alternative polyadenylation. ~12% of the EST sequences matched the 3' ends of the corresponding mRNAs, the remainder matching the 5' ends. We identified ~470 candidate cDNAs for further indexing. These restrictions minimize potential concerns about multi-gene families and avoid artifacts from contaminants in the EST libraries . As a positive control for the method, vimar shows two alternatively polyadenylated transcripts for which the 3'ends in the database are supported by experimental analysis . As expected, queries for sequences from only the region of interest specifically identify this candidate using Fast-FIND.
Confirming the significance of motif co-occurrence
To test whether pairs of binding sites were correlated in abundance, we used the G test to determine whether transcripts that contained one site were more likely to contain the other. The G test is a test for association similar to the familiar chi-squared test widely used in genetics, but is more accurate for small sample sizes . To confirm the G test results, we performed Monte Carlo simulations of the sequences using three Markov models: (a) first-order Markov model using the full set of regions of interest as a training set; (b) fifth-order Markov model using the full set of regions of interest as a training set; and (c) permuting the nucleotides in each sequence independently. The first two of these models preserve the single-base and five-base 'word' frequencies respectively, while the third model accounts for compositional heterogeneity between the sequences. For each model, we made 1000 random sets of sequences of the same length as the actual sequences, recalculated the G statistic using each random set, and compared the value of the G statistic in the actual set to that in each random set. The empirical P-value was estimated as the fraction of random sets with lower G scores than the actual set. This analysis corrects for any error due to biases in the length or composition of the alternatively polyadenylated transcripts. As an additional control, we verified that the counts and locations of motifs identified by Fast-FIND were identical to those found by using Python's built-in string-matching facility.
We have set up a web form allowing searches of arbitrary combinatorial patterns against the portions of the Drosophila cDNAs relevant for alternative end formation and retrieval of relevant sequences at .
Performance characteristics for arbitrary pattern searches
Candidates with desired binding sites adjacent to alternative polyadenylation sites. Identification of cDNAs with potential alternative 3' ends and various patterns – base composition, degenerate, and combinatorial patterns – located between 100 nucleotides upstream of the 3' end of the first EST set through the 3' end of the second EST set. # and ** cDNAs were used as examples for the alignment shown in Figure 4.
Number of cDNAs with potential alternative 3' ends and search patterns
Number of Patterns
Number of cDNAs
A. Base composition
CstF64; U> = 4, G< = 4, A+C = 0 ;length = 8
SXL; U> = 15, G< = 2, A+C = 0 ;length = 17
SXL1; U> = 8, G+A+C = 0 ;length = 8
SXL2; U> = 10, G< = 2, A+C = 0 ;length = 12
B. Degenerate motifs
hnRNP F/H/H' (core); GGGA
hnRNP F/H/H'; GGGGA
C. Combinatorial motifs
CstF64 + SXL
CstF64 + hnRNP F/H/H' (core)
CstF64 + hnRNP F/H/H'
SXL + hnRNP F/H/H' (core)
PSI + hnRNP F/H/H' (core)
Identification of cDNAs containing binding sites for RNA-binding proteins
In eukaryotes, binding sites and biologically relevant targets are known for very few of the several hundred known RNA-binding proteins [21, 27]. We tested whether several well-characterized RNA-binding proteins were significantly associated with one another near alternative polyadenylation sites. For this analysis, we used several RNA-binding proteins for which binding sites have been identified by iterative selection amplification or biochemically: Sex-lethal (SXL) [28, 29], RBP1 , P-element somatic inhibitor (PSI) , Rbp9 , and hnRNP H/H'/F family . The binding sites for SXL and CstF64 are both defined by base composition rather than by exact sequence [28, 29, 34], highlighting the necessity for both composition- and sequence-based searches. The similarities between the U-rich binding sites for RBP1 (and SXL) and a subset of the GU-rich polyadenylation enhancers or CstF64 binding sites suggested to us that they might affect alternative polyadenylation [9, 12, 13]. SXL regulates specific 5' and 3' splice sites by blocking the binding of a specific splicing factor, U2AF, . Thus it might similarly regulate alternative polyadenylation by binding to and blocking certain GU-rich polyadenylation enhancers, thus activating an alternative polyadenylation site.
Searching for SXL-regulated polyadenylation: a natural compositionally-defined site
If an RNA-binding protein such as SXL affects alternative polyadenylation, we would expect to find its consensus binding site close to the first 3' end of alternatively polyadenylated transcripts from the same gene. We used Fast-FIND to search for cDNAs meeting these criteria.
Searching for combinatorial patterns of degenerate binding sites
In natural situations, genes are typically regulated by combinations of binding sites rather than by single binding sites [1–4]. We tested whether the binding sites for several well-characterized RNA-binding proteins occurred in the same alternative polyadenylation region more frequently than chance would predict. Specifically, for each pair of binding sites, we used the G test to determine whether transcripts that contained one site were more likely to contain the other. The G test is a test for association similar to the familiar chi-squared test widely used in genetics, but is more accurate for small sample sizes .
Although the initial G tests indicated that the RNA-binding patterns for CstF64 and the hnRNP H/H'/F family members are significantly associated in the alternatively polyadenylated regions of Drosophila cDNAs (Table 1), the Monte Carlo analysis did not confirm the significance of these associations. In contrast, associations between SXL and CstF64, SXL and the hnRNP core, and PSI and the hnRNP core were marginally statistically significant in the G test but were highly significant (P < 0.005) in all three Monte Carlo simulations, even when heterogeneity in composition and overlap between the sites were accounted for. One cDNA that provides an example of potential regulation by the hnRNP H/H'/F family of RNA-binding proteins is shown in Figure 4B.
We have used Fast-FIND to identify genes that are promising candidates for regulation by alternative polyadenylation. We have demonstrated that Fast-FIND can be used to rapidly search for both individual and multiple patterns within defined regions of sequence that are biologically important, and that the queries are sufficiently fast to support the analyses of many different variations on pattern, which is important when knowledge about the pattern is limited.
The two proteins that we found to be associated near alternative polyadenylation sites, CstF64 and the hnRNP H/H'/F, have previously been linked to polyadenylation site choice [37–40]. They are not known to interact in the cell, and, in this context, it is interesting that their sites are not significantly co-located, although each is significantly co-located with other RNA-binding proteins, including SXL. The other proteins we examined have not been implicated in polyadenylation regulation, but may act as cofactors with CstF64 or hnRNP H/H'/F. We have thus used our new tool to generate novel hypotheses about specific biological interactions that we now plan to test experimentally. Our identification of alternatively polyadenylated mRNAs that contain specific combinations of binding sites thus opens up possibilities for new studies of both gene function and the molecular mechanisms by which polyadenylation site choice regulates genes. Most importantly, because each of the individual short, degenerate binding sites occurs frequently, identification of such candidates would not have been possible without using associations between the different sites.
Fast-FIND offers several advantages over other solutions to the pattern-matching problem. First, except for the one-time investment of computational resources during the indexing phase (database updates are incremental, requiring minimal additional CPU time), the indexed approach eliminates the linear scan for every new pattern. Consequently, many different combinations of search patterns can be evaluated until an experimentally manageable number of potential cDNAs is identified for further analysis. Second, Fast-FIND allows searches for combinatorial patterns. This capability is particularly important because most eukaryotic genes are regulated by a combination of multiple sequence patterns rather than by a single pattern. Third, Fast-FIND is uniquely suited to finding short patterns defined by composition. Because each window has only one composition, and because the compositions are inherently ordered according to the number of each base they contain, we can store the composition for each window in the database and then search for all windows within any arbitrary range of compositions rapidly. In contrast, it is impossible to precalculate matches for all possible regular expressions or for all possible weight matrices, making it difficult to avoid a new O(N) linear scan through the sequence when searching for a new pattern. Finally, Fast-FIND is easily implemented using standard database software, making it easily accessible to a broad audience.
The indexing approach used in Fast-FIND is not appropriate for all applications. The database tables take up much more space than the original sequences, limiting its use for extremely large data sets. Generalized suffix trees and suffix arrays provide better performance in a smaller space in cases where the entire data structure can fit into main memory and concurrent access by multiple users is not required. Weight matrices are more suitable for highly degenerate sequences, where the O(N) in the number of possible matching patterns can become larger than a linear scan that is O(N) in the length of the sequence to be searched. The specific indexing scheme we used for this analysis does not handle degenerate bases, although we provide this capability by using a different bitvector scheme, which degrades the search performance to a linear scan (data not shown). However, even taking these limitations into account, Fast-FIND is useful for a wide range of biologically important problems.
Fast-FIND provides a versatile tool for searching for exact and compositionally defined search patterns. We have applied this approach to analyzing the role of RNA-binding proteins with known binding sites in regulating alternative polyadenylation in Drosophila, and have found several mRNAs that are plausible targets for known RNA-binding proteins. Although the database we created is Drosophila-specific, the general approach described here can easily be extended to the analysis of combinatorial regulation in other species and in other contexts, including the control of transcription, splicing, mRNA stability, and translation.
Availability and requirements
The Fast-FIND interface is available at http://bmf.colorado.edu/fastfind/. The software is available under the GPL by request to the authors.
- Fast-Fully Indexed Nucleotide Database:
- complementary DNAs:
- expressed sequence tags:
- relational database management systems:
- International Union of Pure and Applied Chemistry:
- P-element somatic inhibitor:
- heterogeneous nuclear ribonuclear protein:
We thank Dr. Jens Lykke-Andersen, Dr. Ken Krauter, and Mark Robida for critical comments on the manuscripts, Dr. Rick Osborne for discussions. This work was supported in part by a grant from the National Institutes of Health, the American Cancer Society, and the Butcher Foundation to RS.
- Levine M, Tjian R: Transcription regulation and animal diversity. Nature 2003, 424: 147–151. 10.1038/nature01763View ArticlePubMedGoogle Scholar
- Smith CW, Valcarcel J: Alternative pre-mRNA splicing: the logic of combinatorial control. Trends Biochem Sci 2000, 25: 381–388. 10.1016/S0968-0004(00)01604-2View ArticlePubMedGoogle Scholar
- Buchler NE, Gerland U, Hwa T: On schemes of combinatorial transcription logic. Proc Natl Acad Sci U S A 2003, 100: 5136–5141. 10.1073/pnas.0930314100PubMed CentralView ArticlePubMedGoogle Scholar
- Singh R, Valcarcel J: Building specificity with nonspecific RNA-binding proteins. Nat Struct Mol Biol 2005, 12: 645–653. 10.1038/nsmb961View ArticlePubMedGoogle Scholar
- Friedl JEF: Mastering Regular Expressions. Sebastopol, CA, O'Rilly and Associates; 1997.Google Scholar
- Durbin R, Eddy S, Krogh A, Mitchison G: Biological Sequence Analysis. Cambridge, Cambridge University Press; 1998.View ArticleGoogle Scholar
- Gusfield D: Algorithms on strings, trees and sequences: computer science and computational biology. Cambridge, Cambridge University Press; 1997.View ArticleGoogle Scholar
- Dominski Z, Marzluff WF: Formation of the 3' end of histone mRNA. Gene 1999, 239: 1–14. 10.1016/S0378-1119(99)00367-4View ArticlePubMedGoogle Scholar
- Hirose Y, Manley JL: RNA polymerase II and the integration of nuclear events. Genes Dev 2000, 14: 1415–1429.PubMedGoogle Scholar
- Colgan DF, Manley JL: Mechanism and regulation of mRNA polyadenylation. Genes Dev 1997, 11: 2755–2766. 10.1101/gad.11.21.2755View ArticlePubMedGoogle Scholar
- Manley JL, Takagaki Y: The end of the message--another link between yeast and mammals. Science 1996, 274: 1481–1482. 10.1126/science.274.5292.1481View ArticlePubMedGoogle Scholar
- MacDonald CC, Redondo JL: Reexamining the polyadenylation signal: were we wrong about AAUAAA? Mol Cell Endocrinol 2002, 190: 1–8. 10.1016/S0303-7207(02)00044-8View ArticlePubMedGoogle Scholar
- Zhao J, Hyman L, Moore C: Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis. Microbiol Mol Biol Rev 1999, 63: 405–445.PubMed CentralPubMedGoogle Scholar
- Bentley D: Coupling RNA polymerase II transcription with pre-mRNA processing. Curr Opin Cell Biol 1999, 11: 347–351. 10.1016/S0955-0674(99)80048-9View ArticlePubMedGoogle Scholar
- Yan J, Marr TG: Computational analysis of 3'-ends of ESTs shows four classes of alternative polyadenylation in human, mouse, and rat. Genome Res 2005, 15: 369–375. 10.1101/gr.3109605PubMed CentralView ArticlePubMedGoogle Scholar
- database DNA: [http://www.fruitfly.org/sequence/sequence_db/na_cDNA.dros].
- database EST: [http://www.fruitfly.org/sequence/sequence_db/na_EST.dros].
- Lewis PM, Kifer M, Bernstein AJ: Database and Transaction Processing. Boston, Pearson Addison Wesley; 2002.Google Scholar
- Chen X, Kwong S, Li M: A Compression Algorithm for DNA Sequences and Its Applications in Genome Comparison. Genome Inform Ser Workshop Genome Inform 1999, 10: 51–61.PubMedGoogle Scholar
- Tacke R, Manley JL: Determinants of SR protein specificity. Curr Opin Cell Biol 1999, 11: 358–362. 10.1016/S0955-0674(99)80050-7View ArticlePubMedGoogle Scholar
- Varani G, Nagai K: RNA recognition by RNP proteins during RNA processing. Annu Rev Biophys Biomol Struct 1998, 27: 407–445. 10.1146/annurev.biophys.27.1.407View ArticlePubMedGoogle Scholar
- Kent WJ: BLAT--the BLAST-like alignment tool. Genome Res 2002, 12: 656–664. 10.1101/gr.229202. Article published online before March 2002PubMed CentralView ArticlePubMedGoogle Scholar
- Beaudoing E, Freier S, Wyatt JR, Claverie JM, Gautheret D: Patterns of variant polyadenylation signal usage in human genes. Genome Res 2000, 10: 1001–1010. 10.1101/gr.10.7.1001PubMed CentralView ArticlePubMedGoogle Scholar
- Lo PC, Frasch M: bagpipe-Dependent expression of vimar, a novel Armadillo-repeats gene, in Drosophila visceral mesoderm. Mech Dev 1998, 72: 65–75. 10.1016/S0925-4773(98)00016-1View ArticlePubMedGoogle Scholar
- Sokal RR, Rohlf FJ: Biometry: the principles and practice of statistics in biological research. 3rd edition. New York, W. H. Freeman and Co.; 1995.Google Scholar
- Fast-FIND: [http://bmf.colorado.edu/fastfind/].
- Burd CG, Dreyfuss G: Conserved structures and diversity of functions of RNA-binding proteins. Science 1994, 265: 615–621. 10.1126/science.8036511View ArticlePubMedGoogle Scholar
- Singh R, Valcarcel J, Green MR: Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science 1995, 268: 1173–1176. 10.1126/science.7761834View ArticlePubMedGoogle Scholar
- Sakashita E, Sakamoto H: Characterization of RNA binding specificity of the Drosophila sex- lethal protein by in vitro ligand selection. Nucleic Acids Res 1994, 22: 4082–4086. 10.1093/nar/22.20.4082PubMed CentralView ArticlePubMedGoogle Scholar
- Heinrichs V, Baker BS: The Drosophila SR protein RBP1 contributes to the regulation of doublesex alternative splicing by recognizing RBP1 RNA target sequences. Embo J 1995, 14: 3987–4000.PubMed CentralPubMedGoogle Scholar
- Amarasinghe AK, MacDiarmid R, Adams MD, Rio DC: An in vitro-selected RNA-binding site for the KH domain protein PSI acts as a splicing inhibitor element. Rna 2001, 7: 1239–1253. 10.1017/S1355838201010603PubMed CentralView ArticlePubMedGoogle Scholar
- Park SJ, Yang ES, Kim-Ha J, Kim YJ: Down regulation of extramacrochaetae mRNA by a Drosophila neural RNA binding protein Rbp9 which is homologous to human Hu proteins. Nucleic Acids Res 1998, 26: 2989–2994. 10.1093/nar/26.12.2989PubMed CentralView ArticlePubMedGoogle Scholar
- Caputi M, Zahler AM: Determination of the RNA binding specificity of the heterogeneous nuclear ribonucleoprotein (hnRNP) H/H'/F/2H9 family. J Biol Chem 2001, 276: 43850–43859. 10.1074/jbc.M102861200View ArticlePubMedGoogle Scholar
- Takagaki Y, Manley JL: RNA recognition by the human polyadenylation factor CstF. Mol Cell Biol 1997, 17: 3907–3914.PubMed CentralView ArticlePubMedGoogle Scholar
- Valcarcel J, Singh R, Zamore PD, Green MR: The protein Sex-lethal antagonizes the splicing factor U2AF to regulate alternative splicing of transformer pre-mRNA. Nature 1993, 362: 171–175. 10.1038/362171a0View ArticlePubMedGoogle Scholar
- Sosnowski BA, Belote JM, McKeown M: Sex-specific alternative splicing of RNA from the transformer gene results from sequence-dependent splice site blockage. Cell 1989, 58: 449–459. 10.1016/0092-8674(89)90426-1View ArticlePubMedGoogle Scholar
- Takagaki Y, Seipelt RL, Peterson ML, Manley JL: The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation. Cell 1996, 87: 941–952. 10.1016/S0092-8674(00)82000-0View ArticlePubMedGoogle Scholar
- Takagaki Y, Manley JL: Levels of polyadenylation factor CstF-64 control IgM heavy chain mRNA accumulation and other events associated with B cell differentiation. Mol Cell 1998, 2: 761–771. 10.1016/S1097-2765(00)80291-9View ArticlePubMedGoogle Scholar
- Veraldi KL, Arhin GK, Martincic K, Chung-Ganster LH, Wilusz J, Milcarek C: hnRNP F influences binding of a 64-kilodalton subunit of cleavage stimulation factor to mRNA precursors in mouse B cells. Mol Cell Biol 2001, 21: 1228–1238. 10.1128/MCB.21.4.1228-1238.2001PubMed CentralView ArticlePubMedGoogle Scholar
- Proudfoot NJ, Furger A, Dye MJ: Integrating mRNA processing with transcription. Cell 2002, 108: 501–512. 10.1016/S0092-8674(02)00617-7View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.