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Table 1 GO Categories for the Rank-Invariant Genes

From: Data-driven normalization strategies for high-throughput quantitative RT-PCR

Gene Cellular Component Molecular Function Biological Processes
GAPDH Cytoplasm Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; protein binding Glycolysis
ENO1 -- Phosphopyruvate hydratase activity --
HSPCB Cytoplasm Nitric-oxide synthase regulator activity; nitric-oxide synthase regulator activity Response to unfolded protein; positive regulation of nitric oxide biosynthetic process
ACTB Cytoplasm; cytoskeleton Protein binding; structural constituent of cytoskeleton Cell motility
EEF1A1 Cytoplasm; eukaryotic elongation factor 1 complex GTP binding; protein binding Translational elongation
  1. The GO categories are listed for the five genes in the rank-invariant set that were identified as reasonable controls to be used for normalization of the PMA dataset. The presence of GAPDH and ACTB as controls were not surprising for this dataset. The inclusion of some of the other categories heat-shock protein activity and translation, were more surprising.