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Table 1 S3T Default Rule Set.

From: A score system for quality evaluation of RNA sequence tags: an improvement for gene expression profiling

Score

Source

Condition

-4

Linker (*)

 

-3

mRNAs internally primed

f(x) = 1

-2

 

f(x) < 5 and (N(x) ∩ T) ≠ Ø

10

FL cDNAs, 3'most, poly(A)

f(x) ≥ 10

9

FL cDNAs, 3'most, poly(A)

1 ≤ f(x) < 10

8

FL cDNAs, 3'most

f(x) ≥ 5

7

FL cDNAs, 3'most

1 ≤ f(x) < 5

6

Consensus, 3'most, poly(A)

f(x) ≥ 5

5

Consensus, 3'most, poly(A)

1 ≤ f(x) < 5

4

Alt. poly(A)/splicing, > 1 est

f(x) > 1

3

Consensus, 3'most

f(x) ≥ 1

2

Alt. poly(A)/splicing, 1 est

f(x) ≥ 1

1

FL cDNAs, internal Tags

 

0

 

m(x) > f(x)

-1

 

f(x) = 1

-5

Mitochondrion genome

 

-7

Nuclear genome

 

-6

Vector pZErO-1 (*)

 

-8

  
  1. Rule set used in S3T classification process of public SAGE data. The f(x) represents the absolute frequency for tag x and m(x) the average frequency for tag x, considering all libraries in our gene expression database, the set N(x) represents the tag x neighbor tags (that differ from it by a single nucleotide), the T set represents the highest frequency tags of the library (top 20%). (* – This is applicable only for SAGE data)