Illustration of different tiling densities, and an example pan-genome tiling. Genomes are represented as horizontal lines and probes as colored rectangles. The offset between probes is the distance between the start of one probe and the start of the next. (1a) Three different tiling densities are shown for genome A. The top figure illustrates a gapped tiling, the middle an end-to-end tiling, and the bottom an overlapping tiling. (1b) A pan-genome tiling is shown for two genomes. Genomes A and B are identical except for a small insertion in B, represented by vertical red bars. Solid blue probes are conserved in both genomes, and probes spanning the insertion event are colored by variant. Set H shows the non-redundant set of probes needed to tile the pan-genome including A and B.