Figure 1From: Deep proteogenomics; high throughput gene validation by multidimensional liquid chromatography and mass spectrometry of proteins from the fungal wheat pathogen Stagonospora nodorumPeptide clusters which did not confirm an existing gene model or conflict with an existing gene model in the opposing orientation were compared to grouped blastx HSPs from related dothideomycete proteins. Some of these peptide clusters were linked back to existing gene models as illustrated by Peptide Cluster 1 and Gene 1, which share a homology relationship with Blastx HSP group 1. This provides strong evidence for the reannotation of Gene 1 to become Modified Gene 1. Other peptide clusters could not be linked to existing gene annotations such as Peptide Cluster 2, which provides evidence for the creation of New Gene 2.Back to article page