Figure 3

Summary of version 1 and 2 S. nodorum genes confirmed and modified by peptide support (either by conventional protein database or by 6-frame genome translation-derived peptide matches). Genes were identified as candidates for re-annotation if the 6-frame translated genome-matched peptides indicated: conflicts in annotated coding-exons open reading frames (A); peptide-genome matches residing within annotated introns or untranslated regions (UTRs) (B) or; peptide-genome matches matched to the genome which could be linked back to a gene model via tblastn homology between the genome sequence and selected dothideomycete genomes (C). 47 new gene candidates were identified by a multiple methods: 3 peptide clusters which could not be linked to an existing gene annotation via a tblastn homolog; 29 unassembled read-contigs matching dothideomycete proteins via blastx but not matching S. nodorum proteins and; 15 unassembled read-singletons matching dothideomycete proteins via blastx but not matching S. nodorum proteins.