Deep proteogenomics; high throughput gene validation by multidimensional liquid chromatography and mass spectrometry of proteins from the fungal wheat pathogen Stagonospora nodorum

Table 3 Relative molecular masses of predicted proteins significantly over-represented within the Mascot peptide supported gene annotations of S. nodorum.¹

	Peptide supported genes (all)	Peptide supported genes (v1)	Peptide supported genes (v2)	Unsupported genes	Expected genes	Total Genes
0 to 20 kDa	284	60	224	4495	633	4779
20 to 100 kDa	1536	121	1415	8816	1371	10352
100 to 500 kDa	310	7	303	669	130	979
>500 kDa	4	0	4	2	1	6
TOTAL	2134	188	1946	13982	2135	16116

¹Numbers of peptide supported genes in each category were compared to expected counts from a random sampling of the whole genome via Fisher's exact test at a significance threshold of <0.05.

ISSN: 1471-2105