Figure 4

Set 1 and Set 2 results under all target and nontarget concentrations. The measured target intensities (green) and nontarget intensities (red) and their ratios (blue) at different (a-e) Set 1 target-to-nontarget concentration ratios and (f-j) Set 2 target and nontarget absolute concentrations are drawn against the hybridization temperatures. Also labeled in each figure is the median ratio value for the presented subset of data in that figure. In these figures, boxes enclose values within the lower and upper quartiles, circled dots indicate median values, and whisker lines extending from the ends of boxes show the extent of the rest of data. To avoid clutter, outliers are not plotted. In these figures temperatures are categorical data and are therefore not plotted to scale on the x-axis.