Figure 2
The percentage of "outlier" spots, P
out
, is plotted versus the intensity class and PMT gain. Results for slide #3. The percentage of "outlier" spots in each intensity class 
is Pc
out
= Nc
out
/Nc, with Nc
out
being the number of events where a spot in the intensity class, c, is found to be an "outlier" between two successive scans and Nc= (Nscans -1) × nc
spots
(Nscans is the number of scans in the series and nc
spots
is the number of spots in the intensity class c). The line with the lozenges is obtained for PMT gains 400-400 (Cy5-Cy3); the line with the squares is for PMT gains 500-500; the line with the triangles is for PMT gains 600-600 and the line with the dots is for PMT gains 700-700. Part A is for the Cy3 image (532 nm); Part B for the Cy5 image (635 nm).