Figure 4
The percentage of "outlier" spots, P
out
, is plotted as a function of the adjusted foreground intensity. Results for slide #3. The percentage of "outlier" spots in each intensity class, 
, is calculated as follows Pc
out
= Nc
out
/Nc, with Nc
out
being the number of events where a spot, in the intensity class, c, is found to be an "outlier" between two successive scans and Nc= (Nscans -1) × nc
spots
(Nscans is the number of scans in the series and nc
spots
is the number of spots in the intensity class). The value of the signal that is used to select the "outlier" spots is the average of the adjusted foreground signals obtained for 1, 2 or 3 acquisitions per line. The grey lines correspond to the Cy3 image (532 nm) and the black lines to the Cy5 image (635 nm). The lines with the circles correspond to one acquisition per line; those with squares to two lines averaged and those with triangles to three lines averaged.