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Table 3 The exceptions to the basic cleavage rules of the trypsin are shown: AA-1 is the amino acid immediately preceding the K or R amino acid, AA0 is the last amino acid before the cleavage site (K or R) and AA1 is the first amino acid after the cleavage site. In these cases, trypsin does not easily cut the amino-acid chain (Source [23]).

From: A Perl procedure for protein identification by Peptide Mass Fingerprinting

AA -1

AA 0

AA 1

not W

K

P

not M

R

P

C

K

D

D

K

D

C

K

H

C

K

Y

C

R

K

R

R

H

R

R

R

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BMC Bioinformatics

ISSN: 1471-2105

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