Table 3 Success rate (%) of data analysis methods when varying the reference sample size; mtDNA sequences were simulated for two species and a speciation time of T = 500 (N _f /2); the mutation parameter θ was either 3 (A) or 30 (B).

(A) θ = 3

3

77.45

77.50

78.05 *

77.15

77.35

76.15

5

84.20 *

83.85

83.30

82.95

82.40

82.10

10 †

87.25*

86.30

87.20

87.15

86.40

87.15

25

92.00 *

91.70

91.10

90.80

89.40 ¶

90.75

1

1

(B) θ = 30

3

82.80

82.95

83.55 *

79.40 ¶

81.95

80.45 ¶

2

2

5

89.50

89.45

90.25 *

86.20 ¶

89.30

86.85 ¶

2

2

10 †

96.10

96.20 *

95.55

93.50 ¶

95.25

94.00 ¶

1

1

25

98.95

98.95

99.15 *

98.30 ¶

99.00

98.20 ¶

2

1