Table 1 Examples of normalization options
From: web cellHTS2: A web-application for the analysis of high-throughput screening data
Normalization option | Description |
|---|---|
Median | Measurements are divided by the median of all sample wells in the plate |
Shorth | The midpoint of the 'shorth' of the distribution of all sample wells is used for normalization |
Mean | Measurements are divided by the mean of all sample wells in the plate |
Negatives | Measurements are divided by the median of negative controls in the plate |
Percent control | Measurements are divided by the mean of the plate's positive control |
Normalized percent control | Measurements are divides by the difference of the plates positive and negative controls |
B-score | A two-way (row and column) median polish is applied to each plate |
Robust local fit regression | Spatial effects are normalized by fitting a bivariate local regression |
Loess regression | Spatial effects are normalized using Loess regression |