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Table 7 Neuroblastoma data: Variability of log geometric means based on optimal gene subsets identified by various methods

From: Selection of optimal reference genes for normalization in quantitative RT-PCR

Set Size Method Optimal set Variance logGM Std Dev logGM
2 Vand GAPDH, HPRT 0.327 0.572
2 Szabo GAPDH, SDHA 0.374 0.612
2 New GAPDH, YWHAZ 0.250 0.500
3 Old1 GAPDH, HPRT, SDHA 0.348 0.590
3 New ACTB, GAPDH, YWHAZ 0.255 0.505
4 Old1 GAPDH, HPRT, SDHA, UBC 0.361 0.601
4 New ACTB, B2M, GAPDH, TBP 0.231 0.480
5 Old1 GAPDH, HPRT, SDHA, UBC, HMBS 0.358 0.598
5 New ACTB, B2M, GAPDH, HPRT1, RPL13A 0.224 0.473
6 Old1 GAPDH, HPRT, SDHA, UBC, HMBS, YWHAZ 0.319 0.565
6 New ACTB, GAPDH, B2M, HPRT1, TBP, YWHAZ 0.227 0.477
  1. 1Same results using either the method of Vandesompele et al [4] or Szabo et al [9]