Figure 4
From: Colonyzer: automated quantification of micro-organism growth characteristics on solid agar
Local thresholding algorithm for fast, sensitive first-pass image segmentation. A) 16 spotted cultures from an example original plate image, B) Sobel gradient map of panel A, C) Mask of top 5% intensity gradients from panel B, D) Mask applied to original image (background set to white) E) Original image intensities locally thresholded by tile to exclude the darkest 33% of pixels from panel D in each tile location