Figure 12
Shape and volume dynamics of the soma of a migrating cell. A and B show three dimensional representations of successive SICM recordings at a time interval of 11 min and the corresponding boundary delimitation approximations of the cell soma. Color bars indicate height and the contribution to the entire soma volume per pixel, respectively. C shows the corresponding widths, lengths and heights of the cell soma. Whereas the width remained constant, the length decreased and the height increased. D shows the corresponding soma volumes, subdivided into frontal and rear part with respect to the direction of migration at the level of C90. Note the swelling of about 140 pL. E shows the size of the area covered by the cell soma. Note that in contrast to the cell soma volume, the area covered by the soma slightly decreased. F shows the ratios of the determined geometrical parameters illustrating their changes between the two scans.