Figure 3
Representation of the location of the nucleus by C 90 . A and B show light microscopic images from an oligodendrocyte precursor cell whose nucleus was stained using Hoechst 33342 (B) and that was scanned by backstep SICM (C). The position of the scan is depicted in A and a three dimensional representation of the data obtained by SICM is shown in C. The positions of C T for varying T (between 10% and 90% of the maximal z-value) calculated from the SICM data with respect to the position of the centroid of the stained area (obtained from fluorescence microscopy as shown in B, marked by the red cross-hair) are drawn rotated and magnified in D (blue dots and blue cross, labels indicate T in percent). E shows the average distances between C T and the centroid of the staining of the nucleus obtained from 3 different determinations, error bars indicate ± SD.